Issue
I need to link a translation to my Benchling-supported standard or nonstandard DNA sequence with an alternate start codon.
Environment
Molecular Biology suite
Cause
Benchling can't automatically determine if a start codon should translate to an alternate amino acid, which may be possible with some nonstandard genetic code tables (ex. Table 11, bacteria + archaea). You need to specify that the translation is using an alternative start codon (methionine).
Resolution steps
- Click Features, then select Translation to navigate to the translation(s) using an alternative start codon.
- Check First codon: Translate as methionine to designate that the translation is using an alternative start codon.