Why register entities individually or in bulk?
You can register plasmids in two ways: single plasmid registration or in bulk.
- The single registration process works well for registering 3 or fewer entities. You'll import your entity, fill out metadata, register, and then create new batches. This is the focus of this article.
- The bulk process works well if you need to register many entities. You'll import metadata and batch information, import plasmid maps, and then register.
Create an entity
We'll walk though an example registration for a plasmid file.
Open a project folder. Toggle to the Inventory Section.
Click “+” and Import DNA. Drop in your plasmid file. Note that we first import all entities in the Inventory before promoting them to the registry.
Toggle to the Metadata Tab and set schema to Plasmid. Depending on your organization's preferences, the 'Plasmid' schema name might be slightly different (i.e. Construct, "Type A" Plasmid).
Fill in values for each field on the Metadata tab.
There are a few fields that are Part Links. Select Autofill Part Fields to link any Part links to your plasmid map. Identified parts will show up as links. See below for how you can create new parts straight from your plasmid map.
Register your entity
If everything looks good, hit the Register button at the top of the metadata page.
In the Register modal, you'll see a few options for generating your ID.
- Generate new registry IDs: Benchling will keep the name of your files, but also give you a registry ID. Note that all names must be unique so you'll hit an error if the file name already exists in the registry.
- Generate new registry IDs based on entity names: Benchling will generate an ID based on the entity name (e.g. DNA002 would be converted to p002). You likely won't use this option.
- Generate new registry IDs and replace name with registry ID: Use this option if you want to make the Registry ID the Name of the entity. Benchling will give you the next ID and then replace the name with the ID. Use this option if you're using a "throwaway" name for your entity.
Depending on the configuration for the 'Plasmid' schema, you will either be able choose from one of the above options or be limited to only one pre-designated option.
Create and register a batch for a single entity
Each entity in Benchling can hold multiple batches. These represent the actual samples of your constructs. For example, after you've done a Maxi prep, you'll want to create an actual batch of a plasmid.
To do so, search and open the Construct entity.
Toggle to the Batches tab and click on the "+" sign next to Batch. Fill out the appropriate information.
You can now use this batch ID on your tube. Remember that you can also @-mention batches in your Notebook.