Benchling supports four different methods of importing DNA. They all begin by clicking the Create button on the main left control panel.

After clicking Create, hover over Sequence and select from File/Database. You’ll have 4 options to import a DNA sequence into Benchling and save it to any project or folder.

Importing From your Computer

Click here to download pBR322 to your computer to practice importing a file into Benchling.

  1. Select the folder and project on Benchling where you would like your sequence to be saved.
  2. Drag and drop a file or click the Choose a File button to locate the file on your computer. We also support importing zip files or selecting multiple files at once to import multiple sequences simultaneously.
  3. Use the menu that appears to adjust sequence properties.

After uploading your file, use the menu that appears to change the file location, sequence topology, or tags associated with the file.

Importing from a Database

Benchling let’s you import DNA sequences from external databases by simply typing in the name of the gene.

You can even specify different ID’s depending on the place you want to import the sequence from. We support the following inputs for pulling DNA from external databases.

DatabaseBenchling Supported Shortcut  NCBI Accession Number Addgene URL Ensembl Ensembl ID iGEM Registry BioBrick ID Joint BioEnergy Institute JBEI ID 

Importing from a Genomic Locus

Benchling supports 20 preloaded genomes. Import DNA from any of these genomes by specifying the locus. You’ll be able to choose the chromosome and exactly which base pairs to pull from the genome.

Inputting a Raw Sequence

Directly input a sequence into Benchling. You can paste a sequence directly from your computer’s clipboard into the window or type it out manually. Specify the topology of your sequence as linear or circular.

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