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  4. Cloning and assembly wizards

Cloning and assembly wizards

  • Running Gibson and Golden Gate assemblies with the combinatorial assembly tool
  • Clone by copy and paste
  • How do I open the Assembly Wizard?
  • Clone using the Digest and Ligate Assembly Wizard
  • Can I perform a Gibson assembly with fragments that are already digested?
  • Clone using the Gibson Assembly Wizard
  • What is the ideal annealing temperature and primer melting temperature (Tm) for Gibson assembly?
  • Clone using the Golden Gate Assembly Wizard
  • How does the Gibson Assembly Wizard factor in 5' and 3' overhangs?
  • Assembly Wizard FAQ
  • Can I maintain restriction sites when using the Gibson Assembly Wizard?
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