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CRISPR

  • Why am I getting the error "Distinct type IIS enzyme cut sites were not found at the specified start and end"?
  • How are on-target scores calculated?
  • Design guide RNAs (gRNAs)
  • How are off-target scores calculated?
  • Why are my paired nickase scores lower than my single guide scores?
  • Assemble guide RNAs (gRNAs) into plasmids
  • Why can I only see a finite number of off-target sites?
  • Design homologous recombination (HR) templates
  • CRISPR FAQ
  • How do I set the genomic region of my imported sequence?
  • Export a table of on-target or off-target scores and sites from your CRISPR experiment
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