See Configure the Rows of the input file generator for information on generating the rowConfigs object.
Overview
Plate layout specific transfers can be configured within a Connect run to maintain the correct plate order and well position without consolidating all source wells into the minimum destination plates.
There are key requirements to set up your Plate Layout successfully:
- When using Plate Layout, both the Source and Destination need to be plates (as opposed to the more common designation at the well level).
- Destination plate(s) need to be specified under Destination
- the Plate Layout removes the need to filter empty wells
Example using the Run Configuration UI
Input file configurations:
- Configure the display name. This will be the section header for the input file in the run.
- Click on the “+” sign to specify Sources and Destinations plates.
- Configure columns for the input file using the Input table.
- Select the Destination and Plate Layout.
- Right click on the Input table header and insert two columns.
- Set lookup for the first column to lookup the source container and the second column to lookup the destination container.
Output file configurations:
- Configure the display name. Tip: Use the same display name as the input file to generate and send the file directly in the Run .
- Select Transfer samples under Benchling actions.
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Configure column mapping to tell Benchling how to interpret the columns in your output file.
- Map the Source Container column to Source Container Barcode from the Source dropdown.
- Map the Destination Container column to Destination Container Barcode from the Destination dropdown.
Types of Plate Layouts in Lab Automation
There are a few Plate Layout types that can be chosen: Column interleave, Interleave, Reverse column interleave, Reverse interleave, Reverse stamp to quadrants, Stamp, and Stamp to quadrants.
Column interleave
Runs can be configured to interleave 4x source plates into a designated plate by column. The Column interleave accounts for partially filled plates and will preserve empty and filled locations as seen in the source plate.
Interleave
Alternatively, Connect runs can be configured to interleave 4x source plates into a designated plate.
Reverse column interleave
Use Reverse column interleave to reverse interleave a single source plate into 4x destination plates by Column. It accounts for partially filled plates and will preserve empty and filled locations as seen in the source plate.
Reverse interleave
Connect runs can be configured to reverse interleave a single source plate into 4x destination plates by column. The Reverse interleave accounts for partially filled plates and will preserve empty and filled locations as seen in the source plate.
Stamp
Source plates often have unique plate mappings that must be stamped into an identical layout. The Stamp Plate Layout accounts for partially filled plates and will preserve empty and filled locations as seen in the source plate.
Stamp to quadrants
Source plates are often transferred to a more scalable plate during processing to enable high throughput functions. Connect runs can be configured to stamp each source plate into a designated quadrant of a 4x destination plate. The Stamp to quadrants Plate Layout accounts for partially filled plates and will preserve empty and filled locations as seen in the source plate.