When I click high-fidelity enzymes, why are other restriction enzymes marked unavailable?


When you click high-fidelity (HF) to search for HF enzymes, this masks enzymes that do not have a high-fidelity version. This does not mean that there are no cut sites for these enzymes, only that there isn't a HF version. 

To see all enzyme options, toggle between checking and unchecking high-fidelity.

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