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Assembly Wizard FAQ

Aarthi
Aarthi
  • Updated October 23, 2022 07:01

How do I open the Assembly Wizard?

Can I perform a Gibson assembly with fragments that are already digested?

What is the ideal annealing temperature and primer melting temperature (Tm) for Gibson assembly?

How does the Gibson Assembly Wizard factor in 5' and 3' overhangs?

Can I maintain restriction sites when using the Gibson Assembly Wizard?

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  • How do I open the Assembly Wizard?
  • Can I perform a Gibson assembly with fragments that are already digested?
  • Can I maintain restriction sites when using the Gibson Assembly Wizard?
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  • What is the ideal annealing temperature and primer melting temperature (Tm) for Gibson assembly?
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