Why are my paired nickase scores lower than my single guide scores?

Aarthi
Aarthi

We calculate paired nickase scores using the same calculations as crispr.mit.edu

Paired scores should be compared relative to other paired scores, while the single guide scores should be compared to other single guide scores. It is a bit unintuitive that the score is lower for the paired compared to single, considering that the nickase pair will have a lower off-target cut probability overall biologically.

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