Using disease induction date for plotting and analysis

Judi
Judi
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Understanding In Vivo dates

 

Term

Definition

Usage

Tracking Date

The date from which Benchling In Vivo starts tracking measurements for visualization and analysis. Typically, this is the date of the first treatment or randomization. It is set individually for each animal to support staggered enrollment.

  • Measurements taken on the tracking date serve as baseline data for percentage change calculations and further analysis.

  • Provides flexibility to start tracking from the first measurement or a later point (e.g., when tumors reach a specific volume).

  • Assign via the animals screen by selecting animals, clicking bulk action, and setting the tracking date.

  • Note: The tracking date must be set for each animal in a group to include them in graphs, analysis, and group summary data exports, including the Prism report.

Disease Induction Date

The date when the disease or symptoms being researched were induced in the animal (e.g., implantation date). This is set individually for each animal to support staggered enrollment.

  • Assign via the animals screen by selecting animals, clicking bulk action, and setting the disease induction date.

  • Data from this day are compared to the data on the tracking date, establishing the baseline for comparison between initial measurements and subsequent data points.

  • Graphs can plot data as “Days Post Disease Induction,” and measurements before the tracking date can be either included or excluded in graphs and exports..

  • This setting is applied from the Treatment groups page in a study.

Study Start Date

The date marking the beginning of the study, referred to as "Day 1" in tasks. This is auto populated as todays date at study creation and is used only for task scheduling purposes.

  • Used to calculate study day for setting up conditional tasks based on the study start date.

  • Tasks created on the study day will update automatically if the study start date changes.

  • Useful for scheduling and rescheduling tasks and handling recurring tasks.

 

Data analysis Example

 

Example Study Setup

  • Study Start Date: June 25, 2024 (Baseline health checks begin)

  • Disease Induction Date: July 1, 2024 (Tumor implantation event)

  • Tracking Date: July 15, 2024 (Randomization and treatment begins)

How Each Date is Used

  1. Study Start Date:

    • Purpose: Marks the official beginning of the study.

    • Usage: Used for scheduling tasks such as initial animal setup, baseline measurements, and recurring health checks.

    • Example:

      • June 25, 2024: Study starts, and tasks like animal housing and baseline health checks are scheduled.

  2. Disease Induction Date:

    • Purpose: Records the date when the disease was induced in the animals.

    • Usage: Used as a reference point in data analysis to compare against the tracking date.

    • Example:

      • July 1, 2024: cells are implanted into the mice. This date is used later to calculate the days post disease induction for graphing and analysis.

  3. Tracking Date:

    • Purpose: Marks the start of tracking measurements after animals are assigned to study groups and receive first treatment, reference baseline for % change and analysis calculations.

    • Usage: All measurements taken on this date are set as the baseline for further group comparisons. This MUST be assigned to all animals to be included in graphs and exports.

    • Example:

      • July 15, 2024: The first measurements (Day 0 data) are taken; tracking of study groups begins from this point.

Analysis Example

To calculate Tumor Growth Inhibition (TGI) at Disease Induction Day 20:

  • Disease Induction Day 20: July 21, 2024 (20 days post-induction from July 1, 2024).

  • Tracking Day Equivalent: July 21, 2024 is Tracking Day 6 (since tracking started on July 15, 2024).

  • Procedure:

    1. Measure tumor volumes on July 21, 2024 (Disease Induction Day 20 / Tracking Day 6).

    2. Compare these volumes to the baseline measurements from July 15, 2024 (Tracking Day 0 / Disease induction Day 14)

 

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Plotting based on days post disease induction

To change the x-axis to reflect days post disease induction and preset data, measurements, dosing etc. in reports according to the days post disease induction follow the steps below:

 

  • Navigate to the Treatment groups page in your study
  • Change the Days Plotted setting to "Post disease induction date" 
  • If you would like to see all data from the disease induction date displayed in the graph keep the check box for "exclude data before tracking date" unchecked. However, If you would like to only see and export data after the tracking data but keep a record of the days as days post disease induction then check this box.

Screenshot 2024-10-22 at 17.40.09.png

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  • Go to the graphs page in the study 
  • The x-Axis title should now show is this is a study day post disease induction or post tracking date. 

Screenshot 2024-10-22 at 17.39.57.png

This will be maintained in exports including the Graphpad prism report. 

 

Caveats and Limitations

  • This is not included in study design or study cloning. Once the study has been created the user will update the setting in the Treatment groups page
  • You must have write access to the study to apply or change this setting. 

 

 

 

 

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