Opening the combinatorial assembly tool

Sakura
Sakura
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You can model Gibson, Golden Gate, concatenation and Homology based assemblies and design constructs in bulk using the combinatorial assembly tool. This article explains how to access the tool.

Gibson, Golden Gate and Homology assemblies

You can access the cloning tool for Golden Gate and Gibson assembly from the Assembly Wizard or global create menu.

Assembly Wizard

  1. Open a DNA sequence entity and click the Sequence Map tab.
  2. Click Assembly Wizard in the bottom-right corner of the screen. 
  3. Select the Gibson or Golden Gate option
  4. Click Try Benchling's new tool here in the blue notification that displays in the assembly modal.

You can access the cloning tool for Golden Gate, Gibson and Homology assembly from the global create menu.

Global create menu

  1. Click the + icon in the Home menu.
  2. Under Assembly select Assemble DNA sequences by cloning
  3. Select the Gibson, Golden Gate or Homology option
  4. Set number of Fragment bins 
  5. Set Linear or Circular Typology 

Concatenation assemblies

You can access the cloning tool for concatenation assemblies from the global create menu.

Global create menu

  1. Click the + icon in the Home menu.
  2. Under Assembly, select Assemble sequences and oligos by concatenation
  3. Select your Sequence type - DNA, RNA or AA 
  4. Set Construct type and Typology when applicable 
  5. Set number of Fragment Bins 
  6. Click Save



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