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Assembly Wizard FAQ

Assembly Wizard FAQ

Get answers to frequently asked questions about the Benchling Assembly Wizard.
Lily Helfrich avatar
Written by Lily Helfrich
Updated over a week ago

How do I open the Assembly Wizard?

Can I perform a Gibson assembly with fragments that are already digested?

What is the ideal annealing temperature and primer melting temperature (Tm) for Gibson assembly?

How does the Gibson Assembly Wizard factor in 5' and 3' overhangs?

Can I maintain restriction sites when using the Gibson Assembly Wizard?

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