The pI (isoelectric point) refers to the pH at which the net charge of a protein is zero. We calculate the theoretical pI by using the pKa's of amino acids and summing the net charge across the protein at a given pH, searching with our algorithm for the pH at which the net charge is zero.
How is a protein's isoelectric point (pI) calculated?

Written by Damian Costello
Updated over a week ago
Updated over a week ago