Off-target scores are calculated based on Hsu et al. 2013

If you have not provided a genomic region, scores may differ from other sites (such as crispr.mit.edu). 

Use these scores to rank guides relative to each other. Scores range from 0-100, with higher being better. Off-target scores above 50 are considered to be good guides. (For more detail, see crispr.mit.edu/about#score.)

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